Dr. Manoj Kumar Singh
Senior Scientist
manoj[dot]singh[at]icar[dot]gov[dot]in
Address :
Animal Biotechnology Centre, ICAR-NDRI, Karnal
At present there is no content available for this section, once content will be available would be updated.
- Embryo Biotechnology, Somatic Cell Cloning, Stem Cell Biology, Transgenesis, Ovum pick-up.
| Sr. No. | Degree | Name of the College/Institute and University | Passing Year |
|---|---|---|---|
| 1 | Ph.D. (Animal Biotechnology) | ICAR-N.D.R.I., Karnal | 2009 |
| 2 | M.V.Sc. & A.H. (Veterinary Biotechnology) | Biotechnology Centre, J.N.K.V.V., Jabalpur | 2004 |
| 3 | B.V.Sc. & A.H. | Bombay Veterinary College, Mumbai/M.A.F.S.U., Nagpur | 2002 |
Present Lab Members: Dr. M.K. Singh & Dr. P. Palta
Students currently enrolled
| Sr. No. | Name of Student | Class | Dissertation Topic |
|---|---|---|---|
| 1 | Mr. Gaurav Tripathi (15-P-BT-9) | Ph.D. Scholar | Effect of miRNA-29b and miRNA-449b on buffalo transgenic embryos, containing human insulin gene, produced by handmade cloning. |
| 2 | Ms. Tanya Gupta (16-P-BT-06) | Ph.D. Scholar | Global DNA methylation profile of spermatozoa of cloned buffalo bulls and their respective donor bulls. |
| 3 | Ms. Kumari Rinka (16-P-BT-07) | Ph.D. Scholar | Effect of trichostatin A and 5-aza-2’-deoxycytidine on the developmental competence and transcriptome profile of buffalo embryos produced by Hand-made cloning. |
| 4 | Ms. Sonal Gupta (17-P-BT-13) | Ph.D. Scholar | Effect of oxamflatin and ascorbic acid on the developmental competence and transcriptome profile of buffalo embryos produced by Hand-made cloning (Proposed). |
| 5 | Mr. Atul Kaushal (18-P-BT-11) | Ph.D. Scholar | Course work |
| 6 | Mr. Prem Kumar (19-P-BT-13) | Ph.D. Scholar | Course work |
| 7 | Mr. Faiz Akram (16-M-BT-06) | M.Sc. Scholar | Course work |
| 8 | Ms. Smriti Gupta (18-M-BT-09) | M.Sc. Scholar | Course work |
| 9 | Ms. Kanika Gandhi (16-M-BT-02) | M.Sc. Scholar | Course work |
| 10 | Ms. Ritika (19-M-BT-10) | M.Sc. Scholar | Course work |
At present there is no content available for this section, once content will be available would be updated.
| Sr. No. | Title | Authors | Year/Journal |
|---|---|---|---|
| Articles in Foreign Referred Journals (39) | |||
| 1 | Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions. | Sharma A, Shah SM, Tiwari M, Roshan M, Singh MK, Singla SK, Palta P, Manik RS, Chauhan MS. | 2020, Cytotechnology, 72:489-497. |
| 2 | Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients. | A. Sharma, A. Kumaresan, P. Mehta, N. Nala, M.K. Singh, P. Palta, S.K. Singla, R.S. Manik. | 2020, Theriogenology, 142:441-449. |
| 3 | Media switching at different time periods affects the reprogramming efficiency of buffalo fetal fibroblasts. | N. Rawat, M.K. Singh, T. Sharma, P. Vats, D. Nagoorvali, P. Palta, M.S. Chauhan, R.S. Manik. | 2019, Animal Biotechnology, DOI: 10.1080/10495398.2019.1671435 |
| 4 | Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization. | S.V. Lagah, T.J. Sood, P. Palta, M. Mukesh, M.S. Chauhan, R.S. Manik, M.K. Singh, S.K. Singla. | 2019, Cellular Reprogramming, 21(4):200-209. |
| 5 | Calcium ionophore enhanced developmental competence and apoptotic dynamics of goat parthenogenetic embryos produced in vitro. | Dua D., Nagoorvali D., Chauhan M.S., Palta P., Mathur P., Singh M.K. | 2019, In Vitro Cell Dev Biol Anim., 55(3):159-168. |
| 6 | Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT. | P. Mehta, R. Kaushik, K.P. Singh, A. Sharma, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla, R.S. Manik. | 2019, Theriogenology, 135, 25e32. |
| 7 | Supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (Bubalus bubalis) spermatogonial stem cells by upregulating the expression of mir-20b, mir-21, and mir-106a. | A. Sharma, S.V. Lagah, D. Nagoorvali, B.S.B. Kumar, M.K. Singh, S.K. Singla, R.S. Manik, P. Palta, M.S. Chauhan. | 2019, Cell Reprogram., 2019 Feb;21(1):11-17. |
| 8 | Optimization of serum-free culture conditions for propagation of putative buffalo (Bubalus bubalis) spermatogonial stem cells. | A. Sharma, S.M. Shah, N. Saini, P. Mehta, B.S.B. Kumar, D. Dua, M.K. Singh, S.K. Singla, P. Palta, R.S. Manik, M.S. Chauhan. | 2019, Cell Reprogram., 21(1):1-10. |
| 9 | Treatment of buffalo (Bubalus bubalis) SCNT embryos with microRNA-21 mimic improves their quality and alters gene expression but does not affect their developmental competence. | Rashmi, S. Sah, S. Shyam, M.K. Singh, P. Palta. | 2019, Theriogenology, 126, 8-16. |
| 10 | Epigenetic alteration of donor cells with histone deacetylase inhibitor m-carboxycinnamic acid bishydroxymide (CBHA) improves the in vitro developmental competence of buffalo (Bubalus bubalis) cloned embryos. | Agarwal H, Selokar NL, Saini M, Singh MK, Chauhan MS, Palta P, Singla SK, Manik RS. | 2018, Cell Reprogram., 20(1):76-88. |
| 11 | m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos. | Agarwal, H., Selokar, N.L., Saini, M., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. and Manik, R.S. | 2018, Reproduction in Domestic Animals, Doi:10.1111/rda.13198. |
| 12 | Establishment, growth, proliferation and gene expression of buffalo (Bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by hand-made cloning using these cells. | Mehta, P., Kaushik, R., Singh, K.P., Sharma, A., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. and Manik R.S. | 2018, Cellular Reprogramming, 20(2): 135-143. |
| 13 | Testicular cell-conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte- and oocyte-like cells. | Shah SM, Saini N, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. | 2016, Theriogenology, 86 (3): 715-729. |
| 14 | Effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (Bubalus bubalis) embryos produced by hand-made cloning. | Sandhu A, Mohapatra SK, Agrawal H, Singh MK, Palta P, Singla SK, Chauhan MS, Manik RS. | 2016, Cell Reprogram, 18(5):356-365. |
| 15 | Supplementation of tauroursodeoxycholic acid during IVC did not enhance in vitro development and quality of buffalo IVF embryos but combated endoplasmic reticulum stress. | A. Sharma, H. Agrawal, N. Mullani, A. Sandhu,M.K. Singh, M.S. Chauhan, S.K.Singla, P. Palta, R.S. Manik. | 2015, Theriogenology 84 (2015) 200-207. |
| 16 | Caspase-9 inhibitor Z-LEHD-FMK enhances the yield of in vitro produced buffalo (Bubalus bubalis) pre-implantation embryos and alters cellular stress response. | Mullani N, Singh MK, Sharma A, Rameshbabu K, Manik RS, Palta P, Singla SK, Chauhan MS. | 2015, Research in Veterinary Science, 104:4-9. |
| 17 | Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization. | Saini N, Singh MK, Shah SM, Singh KP, Kaushik R, Manik RS, Singla SK, Palta P, Chauhan MS. | 2015, Animal, 9(12):1979-1985. |
| 18 | Molecular characterization and expression of buffalo (Bubalus bubalis) DEAD-box family VASA gene and mRNA transcript variants isolated from testis tissue. | Kaushik R, Singh KP, Bahuguna V, Rameshbabu K, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS. | 2015, Gene, 572(1):17-26. |
| 19 | Cumulus cell-conditioned medium supports embryonic stem cell differentiation to germ cell-like cells. | Shah SM, Saini N, Ashraf S, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS. | 2015, Reproduction Fertility and Development, 29: 679-693. |
| 20 | Comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (Bubalus bubalis) ovaries and testes. | Shah SM, Saini N, Ashraf S, Zandi M, Singh MK, Manik RS, Chauhan MS. | 2015, Reproduction in Domestic Animals, 50(3):365-377. |
| 21 | Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System. | Zandi M, Muzaffar M, Shah SM, Singh MK, Palta P, Kumar Singla S, Manik R, Chauhan MS. | 2015, Cell J, 17(2):264-73. |
| 22 | Activation and inhibition of the Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells: effects of WNT3A, Bio and Dkk1. | Zandi M, Shah SM, Muzaffar M, Singh MK, Palta P, Singla SK, Manik RS, Chauhan MS. | 2015, International Journal of Fertility and Sterility, 9(3): 361-70. |
| 23 | Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. | Ashraf S, Shah SM, Saini N, Dhanda S, Kumar A, Goud TS, Singh MK, Chauhan MS, Upadhyay RC. | 2014, Journal of Assisted Reproduction and Genetics, 31(10):1349-1360. |
| 24 | Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them. | Singh K.P., Kaushik R., Mohapatra S.K., Garg V., Rameshbabu K., Singh M.K., Palta P., Manik R.S., Singla S.K., Chauhan M.S. | 2014, Gene Expression Patterns, 16(1):23-30. |
| 25 | Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line. | Kaushik, R., Singh, K.P., Kumari, A., Rameshbabu, K., Singh, M.K., Manik, R.S., Palta, P., Singla, S.K., Chauhan, M.S. | 2014, Molecular Biology Reports, 41(9):5891-5902. |
| 26 | Buffalo (Bubalus bubalis) ES cell-like cells are capable of in vitro skeletal myogenic differentiation. | M.K. Singh, K.P. Singh, D. Kumar, R.A. Shah, T. Anand, M.S. Chauhan, R.S. Manik, S.K. Singla, P. Palta. | 2013, Reprod. Dom. Anim., 48(2):284-91. |
| 27 | WNT3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells. | Zandi M, Muzaffar M, Shah SM, Kaushik R, Singh MK, Palta P, Singla SK, Manik RS, Chauhan MS. | 2013, Reproduction, Fertility and Development, 26: 551-561. |
| 28 | Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro. | A. Yadav, K.P. Singh, M.K. Singh, N. Saini, P. Palta, R.S. Manik, S.K. Singla, R.C. Upadhyay, M.S. Chauhan. | 2013, Reproduction in Domestic Animals, 48(5): 858-865. |
| 29 | Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line. | Kaushik R, Singh KP, Kumari A, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS. | 2013, In Vitro Cellular & Dev Biol – Animal, 49(1): 1-7. |
| 30 | Effects of GDNF, FGF2 and EGF on proliferation and expression of some important genes in buffalo (Bubalus bubalis) spermatogonial cells. | P.H. Kadam, S. Kala, H. Agrawal, K.P. Singh, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla, R.S. Manik. | 2012, Reproduction, Fertility and Development, 25: 1149-1157. |
| 31 | Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic and handmade cloned embryos. | M. Muzaffar, N.L. Selokar, K.P. Singh, M. Zandi, M.K. Singh, R.A. Shah, M.S. Chauhan, S.K. Singla, P. Palta, R.S. Manik. | 2012, Cellular Reprogramming, 14(3):267-79. |
| 32 | In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell. | Kala S, Kaushik R, Singh KP, Kadam PH, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS. | 2012, J. Assist. Reprod. Genet., 29(12): 1335-1342. |
| 33 | Expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (Bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation. | Singh KP, Kaushik R, Garg V, Sharma R, George A, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS. | 2012, Cell. Reprogram., 14(6): 530-538. |
| 34 | Oxygen concentration and cysteamine supplementation during in vitro production of buffalo (Bubalus bubalis) embryos affect mRNA expression of BCL-2, BCL-XL, MCL-1, BAX and BID. | Elamaran G, Singh KP, Singh MK, Singla SK, Chauhan MS, Manik RS, Palta P. | 2012, Reprod. Domest. Anim., 47(6): 1027-1036. |
| 35 | Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells. | Kumar, D., Anand, T., Singh, K.P., Singh, M.K., Shah, R.A., Chauhan, M.S., Palta, P., Singla, S.K., and Manik, R.S. | 2011, J Assist Reprod Genet., 28: 697-688. |
| 36 | Buffalo (Bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. | T. Anand, D. Kumar, M.K. Singh, R.A Shah, M.S. Chauhan, R.S. Manik, S.K. Singla, P. Palta. | 2011, Reprod. Dom. Anim., 6: 50-58. |
| 37 | Effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (Bubalus bubalis) embryos. | S. Saugandhika, D. Kumar, M.K. Singh, R. Shah, T. Anand, M.S. Chauhan, R.S. Manik, S.K. Singla, P. Palta. | 2010, Reprod. Dom. Anim., 45 (5): 931-933. |
| 38 | Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis). | R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta, S.K. Singla. | 2009, Theriogenology, 71(8):1215-9. |
| 39 | Hand-Made Cloned Buffalo (Bubalus bubalis) Embryos: Comparison of Different Media and Culture Systems. | R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta, S.K. Singla. | 2008, Cloning and Stem Cells, 10 (4):435-42. (Now Cellular Reprogramming) |
| Articles in Indian Referred Journals (07) | |||
| 40 | Comparative study of three different media to improve semen processing and flow cytometric analysis in bovine. | Nala N., Kumar V., Singh M.K., Manik R.S. and Singla S.K. | 2017, Journal of Animal Research, 7(3): 537-541. |
| 41 | Derivation, enrichment and characterization of goat (Capra hircus) spermatogonial stem cells from pre-pubertal testes. | Sharma A, Shah SM, Saini N, Kaushik R, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. | 2016, Indian J. Anim. Res., 50(5): 662-667. |
| 42 | ‘Holi’: India’s first cattle calf produced through ovum pick-up – IVF technology- from an aged sahiwal cattle. | Saini N, Singh MK, Singh KP, Manik RS, Singla SK, Palta P and Chauhan MS. | 2015, Indian J. Animal Sci., 85(3), 244-246. |
| 43 | Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation. | Singh, MK, Saw S, Singh KP, Saini N, Kaushik R, Manik RS, Palta P, Singla SK and Chauhan MS. | 2014, Indian Journal of Animal Sciences, 84 (2): 146–151. |
| 44 | Characterization of the coding region of basic fibroblast growth factor (FGF2) c-DNA (open reading frame) in buffalo cumulus cells. | Kumar S, Singh MK, Kumar V, Palta P, Singla SK, Manik RS and Chauhan MS. | 2013, Indian Journal of Animal Sciences, 83(7):744-746. |
| 45 | Expression patterns of OCT-4 and NANOG genes in buffalo (Bubalus bubalis) embryos produced by in vitro fertilization or parthenogenetic activation. | Kumar D, Anand T, Shah RA, Singh MK, Chauhan MS and Manik RS. | 2012, Ind. J. Anim. Sci., 82(8): 834-837. |
| 46 | Generation of buffalo embryonic stem cell-like cells from in vitro produced, day 8 hatched and day 9 expanded blastocysts. | Kumar D, Anand T, Singh KP, Shah RA, Singh MK, Chauhan MS, Palta P, Singla SK and Manik RS. | 2012, Ind. J. Anim. Sci., 82(8): 838-843. |
At present there is no content available for this section, once content will be available would be updated.
At present there is no content available for this section, once content will be available would be updated.
Technologies Developed:
- Ovum pick-up-IVF technique standardised in indigenous cattle (India’s first cattle calf “Holi” produced)
- Hand-guided Cloning protocol in buffalo developed.
- Established the procedure of buffalo embryonic stem cell culture and their induced differentiation into myogenic cells.
- Establishment of spermatogonial stem cell line in buffalo and goat.
- Protocol established for transgenic embryo production in goat and buffalo.
Research Projects
Research Projects handled/being handled:
| Sr. No. | Title of the project | Level of association (PI/Co-PI) | Period From (DD/MM/YYYY) | Period To (DD/MM/YYYY) | Sponsoring organization | Project Cost in Lakhs |
|---|---|---|---|---|---|---|
| On-Going | ||||||
| 1 | Conservation of Indigenous Pig of Assam through Handmade Cloning technique | PI (Collaborative Institute) | 02.01.2017 | 02.07.2020 | DBT, New Delhi | 40.59 Lakh (NDRI) |
| 2 | Global transcriptome and miRNA analysis for deciphering reasons for low cloning efficiency in buffalo | Co-PI | 22.03.2018 | 21.03.2021 | DST, New Delhi | 46.78 Lakh |
| 3 | Production of multiple copies of elite buffalo bulls using animal cloning technology | Co-PI | 01.04.2018 | 31.03.2022 | NASF, New Delhi | 264.3 Lakh |
| Completed | ||||||
| 4 | Synthetic Endometrium: A novel model to study early embryonic development and uterine health in ruminants | Co-PI | 01.04.2017 | 31.03.2020 | NASF (ICAR) | 77.48 Lakhs (NDRI) |
| 5 | Generation of induced pluripotent stem (iPS) cell-like cells from buffalo fibroblasts | PI | 01.04.2014 | 31.03.2017 | N.D.R.I., Karnal (Institute Project) | 27.00 Lakh |
| 6 | Development of parthenogenetic goat from embryonic stem cells | PI (Collaborative Centre) | 01.04.2013 | 31.03.2017 | NFBSFARA (ICAR) | 90.84 Lakhs |
| 7 | Semen sexing in cattle | Co-PI | June 2015 | 31.03.2017 | ICAR, New Delhi | - |
| 8 | Expression of apoptosis-related genes in buffalo embryos produced in vitro under different oxygen concentrations | PI | January, 2010 | Dec, 2012 | N.D.R.I., Karnal (Institute Project) | 49.56 Lakh |
| 9 | Establishment of facilities for research and training in stem cell technology in pigs | Co-PI PI from March 30, 2016 | April, 2012 | October, 2016 | DBT, New Delhi | 37.38 Lakhs |
| 10 | Buffalo transgenesis for expression of desired proteins | Co-PI PI from March 30, 2016 | June, 2014 | June, 2016 | DBT, New Delhi | 59.078 Lakhs |
| 11 | Development of transgenic goat for production of human lactoferrin | Co-PI PI from March 30, 2016 | June, 2012 | May, 2016 | NFBSFARA (ICAR) | 253.68 Lakhs |
| 12 | Characterization and differentiation of embryonic and spermatogonial stem cells in cattle and buffaloes. | Co-PI | Sep. 2008 Joined from July, 2009 | June, 2014 | N.A.I.P., New Delhi | 398.51 Lakhs |
| 13 | Development of transgenes for in vitro expression in buffalo mammary gland epithelial cells | Co-PI | Sep. 2011 | Sep. 2013 | DBT, New Delhi | 43.08 Lakhs |
| 14 | Improvement in fertilizability of cryopreserved buffalo bull semen by minimizing cryocapacitation and apoptosis like changes | Co-PI | March, 2011 | Feb, 2013 | DBT, New Delhi | 17 Lakhs |
| 15 | Molecular cloning and expression of buffalo leukemia inhibitory factor (LIF) in eukaryotic system | Co-PI | January, 2010 | Dec, 2012 | N.D.R.I., Karnal (Institute Project) | 9 Lakhs |
| 16 | Embryo production by Ovum Pick-Up from live cattle | Co-PI | Sep. 2008 Joined from July, 2009 | August, 2011 | DBT, New Delhi | 58.88 Lakhs |
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