Dairy Chemistry
Dairy Chemistry Division is one of the oldest divisions of ICAR-National Dairy Research Institute established in 1941. The mandate of division is to conduct fundamental and applied research for understanding chemistry of milk and milk products, to impart educational programmes for undergraduate and postgraduate courses and to provide R&D support towards chemical-quality control related problems of the dairy industry.
Credited with nearly 32 industrial licenses of different technologies in last five years, Division's work on detection of adulteration in milk and milk products and low cholesterol ghee has won it wide national and international acclaim. Dairy Chemistry division has generated significant quantum of intellectual property (more than 17 in last five years) in addition to quality research publications of high impact factor. The impact of scientific contributions is reflected through recognitions to its faculty which include Fellowships of the national academies, prestigious awards by ICAR, NAAS, NRDC, Professional Societies and research funding from national and international agencies.
Our academic program leading to Master of Science/ Technology and Doctor of Philosophy degrees provides a solid background in dairy chemistry while allowing for specialization in the areas of chemistry of milk constituents, chemistry of milk products, or food chemistry and analytical chemistry. Since our curriculum is grounded in basic science, graduates are able to pursue careers in a wide variety of professional and technical fields.
Division is also supplying a kit for detection of nine different adulterants in milk to various dairy industries on their demand. A wide range of science-based analytical services are offered to food and allied industries through the consultancy cell for compliance under the provision of FSSA 2006, Bureau of Indian Standard (BIS), AGMARK, and other national and international standards. The Faculty from the division is also managing the activities of Chemical Section of National Referral Centre of Milk Quality and Safety. This facility is ISO 17025:2005 certified and has been accredited for more than 175 analytical parameters for chemical testing of milk and milk products.
The division has state of the art air-conditioned Seminar room, Lecture room equipped with interactive board, LCD projectors, Visualiser, Podium etc., Quality control Lab, Undergraduate and Postgraduate labs, Instrument room (equipped with HPLC, GLC, Mastersizer, Ultra filtration, Electrophoresis, Imaging system, Refrigerated water bath, Dual beam digital Spectrophotometer, Water purification system, BOD incubator, Sonicator etc.), Research labs (lipids, proteins and bioactive peptides, minerals, functional foods/ nutraceuticals and quality assurance), Chemical Section of National Referral Centre for Milk Quality and Safety (equipped with FTIR, HPLC, GC-MS, AAS, PCR, Real-time PCR, Particle size analyser, Electrophoresis, Elisa Reader, Western Blot, Digital Refractometer, Protein Analyzer, Lyophilizer, Fume Hoods, Conductivity & Dissolved O2 Meter etc.).
| Sr. No. | Faculty Name | Designation |
|---|---|---|
| 1 | Dr. Sumit Arora | Principal Scientist |
| 2 | Dr. Kamal Gandhi | Scientist |
| 3 | Dr. Bimlesh Mann | Principal Scientist |
| 4 | Dr. Rajan Sharma | Principal Scientist |
| 5 | Dr Rajesh Kumar | Principal Scientist |
| 6 | Dr. Raman Seth | Principal Scientist |
| 7 | Dr. Richa Singh | Scientist |
| 8 | Dr. Vivek Sharma | Principal Scientist |
Academic Programmes
| Sr. No. | Course No. | Title |
|---|---|---|
| 1 | DC-111(2+1) | Physical Chemistry of Milk |
| 2 | DC-122(2+1) | Chemistry of Milk |
| 3 | DC-221(2+1) | Chemistry of milk products |
| 4 | DC-311(2+1) | Chemical Quality Assurance |
| 5 | DC-322(2+1) | Food Chemistry |
| 6 | DC-611(2+1) | Physicochemical aspects of milk and milk Products |
| 7 | DC-612(2+1) | Milk Carbohydrates, Minerals and Water Soluble Vitamins |
| 8 | DC-613(2+1) | Chemistry of Milk Lipids |
| 9 | DC-614(2+1) | Food Chemistry |
| 10 | DC-629(1+0) | Credit Seminar |
| 11 | DC-621(2+1) | Chemistry of Milk Proteins |
| 12 | DC-622(2+1) | Chemistry of milk products |
| 13 | DC-623(2+1) | Chemical Quality Assurance |
| 14 | DC-624(0+3) | Research Techniques |
| 15 | DC-711(3+0) | Advances in Chemistry of Milk Proteins |
| 16 | DC-712(3+0) | Advances in Chemistry of Milk Lipids |
| 17 | DC-713(3+0) | Advances in Chemistry of Functional Dairy Foods & Nutraceuticals |
| 18 | DC-719(1+0) | Credit Seminar |
| 19 | DC-721(3+0) | Advances in the Chemistry of Milk Processing |
| 20 | DC-722(3+0) | Advances in Analytical Techniques in Dairy Chemistry |
| 21 | DC-729 (1+0) | Credit Seminar |
Major areas of Research are:
- Chemistry of milk and milk products for their quality
- Chemical aspects of functional dairy foods /ingredients
Technologies Developed (last five years)
- Technology for the Preparation of High Antioxidant Activity Buffalo Casein Hydrolysates
- Technology for the Preparation of Encapsulated Curcumin for its Applications in Dairy foods
- Technology for the Preparation of Caseinophosphpeptides Enriched Milk Protein Product
- A New Strip based test for detection of Neutralizers in milk
- A New Strip based test for detection of Urea in milk
- Strip based test for detection of glucose in milk
- Strip based test for detection of hydrogen peroxide in milk
- Strip based test for detection of maltodextrin in milk
- A strip based test for detection of sucrose in milk
- A strip for detection of Sodium Chloride in Milk
- Technology to prepare a kit for the detection of Vegetable oil/ fats in ghee
- Methodology to confirm the presence of Lard in ghee
Technologies Commercialized (last five years)
| Sr. No. | Name of the Technology | Inventor | Commercialisation |
|---|---|---|---|
| 1 | New colourbased test for rapid detection of detergent in milk | Rajan Sharma, YS Rajput Amit Kumar Barui | This technology was commercialized to five different commercial houses on 20-03-2013, 19-03-2014, 13-01-2015, 28-4-2018 and 17-7-2018 |
| 2 | Strip based Test for Detection of Neutralizers in Milk | Rajan Sharma, Priyae Brath Gautam, Y. S. Rajput Bimlesh Mann | This technology was commercialized to five different commercial houses on 10-12-2014, 11-12-2014, 14-03-2018, 28-04-2018, 17-07-2018 |
| 3 | Strip based Test for Detection of Added Urea in Milk | Rajan Sharma, YS Rajput, Bimlesh Mann Priyae Brath Gautam | This technology was commercialized to four different commercial houses on 10-12-2014, 11-12-2014, 28-04-2018, 17-7-2018 |
| 4 | Strip based Test for Detection of Hydrogen Peroxide in Milk | Rajan Sharma, YS Rajput, Bimlesh Mann Panchal Bhavesh kumar R | This technology was commercialized to four different commercial houses on 10-12-2014, 11-12-2014, 14-03-2018, 17-07-2018 |
| 5 | Strip based Test for Detection of Glucose in Milk | Rajan Sharma, YS Rajput, Bimlesh Mann Panchal Bhavesh kumar R | This technology was commercialized to three different commercial houses on 10-12-2014, 11-12-2018, 17-07-2018. |
| 6 | Strip based Test for Detection of Maltodextrin in Milk | Rajan Sharma, Y.S. Rajput, Bimlesh Mann Panchal Bhavesh kumar R. | This technology was commercialized to eight different commercial houses on 10-12-2014, 11-12-2014, 09-06-2015, 21-08-2015, 16-09-2015, 13-2-2017, 14-11-2017 and 28-04-2018 |
| 7 | A process for preparation of low cholesterol Ghee | Darshan Lal, Vivek Sharma, Raman Seth, Manoj Kumar Amit Kumar Dairy Chemistry | This technology was commercialized to three different commercial houses on 01-07-2015, 06-04-2016 and 07-06-2016 |
| 8 | A new strip based Test for Detection of Sucrose in Milk | Rajan Sharma, YS Rajput, Bimlesh Mann Priyae Brath Gautam | This technology was commercialized to only one commercial house on 17-07-2018 |
Methods Developed & adopted by FSSAI (last five years)
- Detection of Ammonium Compounds in Milk
- Tests for Presence of Sulphates in Milk
- Detection of Presence of Foreign Fat in Milk
- Detection of Nitrates (Pond Water) in Milk
- Test for Presence of Anionic Detergent in Milk
- Test for Presence of Hydrogen Peroxide in Milk
- Determination of Starch in Khoa
- Detection of Sucrose in Khoa
- Determination of Butyro Refractometer Reading in Ghee
- Method for detection of adulteration in ghee with vegetable oil
Patents Filed (last five years)
| Sr. No. | Title of Patent | Inventors | Date of Filing | Application Number |
|---|---|---|---|---|
| 1 | A strip based detection of added urea in milk and process for the same | Rajan Sharma, Panchal Bhavesh Kumar R. Y. S. Rajput | 29/11/2013 | 3472/DEL/2013 |
| 2 | Aptamer specific for Betacasomorphin 7 (BCM7) | Y. S. Rajput, Abhishek, Rajan Sharma | 18/12/2013 | 3703/DEL/2013 |
| 3 | Casein hydrolysates rich in caseinophosphopeptides | Bimlesh Mann, Prerna, Rajesh Kumar | 05/02/2013 | 331/Del/2013 |
| 4 | Microencapsulated flaxseed oil powder and a method of preparation thereof | Vivek Sharma, Ankit Goyal, Sumit Arora Darshan Lal. | 18/07/2014 | 2030/DEL/2014 |
| 5 | A strip for detection of maltodextrin in milk and process for the same | Rajan Sharma, Y.S. Rajput, Bimlesh Mann, PrernaNarula, Rahul Thakur, Brajesh Kumar | 24/07/2014 | 2097/DEL/2014 |
| 6 | Antimicrobial Nanoemulsion of Clove Oil stabilized with milk protein and a process thereof. | Bimlesh Mann, Minaxi, Rajesh Kumar Rajan Sharma | 31/03/2015 | 913/DEL/2015 |
| 7 | Aptamers specific for cefquinome. | Rajan Sharma, Amit Kumar Barui, Y.S. Rajput, Bimlesh Mann | 15/06/2015 | 1775/DEL/2015 |
| 8 | Oil in Water Curcumin Nanoemulsion and Method of Preparation Thereof | Bimlesh Mann, Rajan Sharma, Rajesh Bajaj | 30/05/2016 | 201611018434 |
| 9 | Caseinophosphopeptides-divalent Metal (Iron/zinc) nanocomplexes and method of preparation thereof | Bimlesh Mann, Prabin Sarkar, Rajan Sharma, Rajesh Bajaj Munmun Sen | 28.03.2017 | 201711010975 |
| 10 | The Preparation of Buffalo Milk Casein Hydrolysates with enriched Antioxidative Peptides and the process thereof. | Rajesh Kumar, Rajeev Kapila, Nichal Mayur Ashok Rao, Sowmya, Gulshan Dass, Bimlesh Mann, Suman Kapila | 04/07/2017 | 201711023424 |
| 11 | An Indicator and the indicator impregnated strip for detection of neutralizers in milk | Rajan Sharma, YS Rajput, GP Brath Bimlesh Mann | 10/08/2018 | 201811030055 |
| 12 | A Preservative formulation for milk and milk product samples stored for analytical purpose | Richa Singh, Mitul, Bimlesh Mann Sumit arora, Priyanka, Divas Pradhan | 9/08/2019 | 201911032383 |
Patents Granted (last five years)
| Sr. No. | Title of Patent | Inventors | Date of Filing | Patent Grant No. | Grant Date |
|---|---|---|---|---|---|
| 1 | A process for the preparation of low cholesterol ghee. (1078/DEL/2006) | DarshanLal, Manoj Kumar, Vivek Sharma, Raman Seth, Amit Kumar | 27/04/2006 | 257783 | 01/11/2013 |
| 2 | A Kit for detection of detergent in milk (1970/DEL/2006) | Y.S. Rajput, Sumandeep Kaur, Rajan Sharma | 04/09/2006 | 264661 | 14/01/2015 |
| 3 | A kit for detection of adulteration of milk with soymilk (396/DEL/2009) | Y.S. Rajput, Rajan Sharma Poonam | 02/03/2009 | 275521 | 08/09/2016 |
| 4 | A multipurpose device for concentration, dialysis and buffer exchange of protein solution and a process for the same (1703/DEL/2006) | Y.S. Rajput M.P. Divya | 25/07/2006 | 276077 | 29.09.2016 |
| 5 | Casein hydrolysates rich in caseinophosphopeptides" (331/Del/2013) | Bimlesh Mann, Prerna Saini Rajesh bajaj | 5/02/2013 | 319794 | 04/09/2019 |
| Sr. No. | Project | Duration | Funding Agency | Working Team | Status of the Project | Total Grants Received (in Lakhs) |
|---|---|---|---|---|---|---|
| 1 | Whey protein-iron complexes: Preparation, characterization and application in biscuits, milk and dahi | 10.3.2017- 09.03.2020 | SERB-DST | Dr. Kamal Gandhi (PI) Dr. Sumit Arora | Continuing | Rs 36.52 |
| 2 | Production of glycomacropeptide from cheese whey at industrial level | 20-8-2018 - 19-8-2021 | SERB-DST | Dr Neelima Sharma(PI) Dr. Rajan Sharma | Continuing | Rs. 31.66/- |
| 3 | Exploring the Dairy Industry Needs for Research and Better Employability of Dairy Graduates | 13.3.2019 - 12.03.2020 | ICAR | Dr. Richa Singh(PI) Dr. Bimlesh Mann Dr. Rajan Sharma | Continuing | Rs.5.5 |
| 4 | Development of Calcium enriched high milk protein powder for convenience formulations of traditional dairy products | 15/10/2019 - 14/10/2021 | MOFPI | Dr. Sumit Arora (PI) Dr. Vivek Sharma Dr. Ashish. K. Singh Dr. G. S. Meena | Continuing | Rs 22.21/- |
| 5 | Development of methods to assess the chemicals migration from the packaging used for dairy products | 15/10/2019 - 14/10/2021 | MOFPI | Dr. Rajan Sharma (PI) Dr. Bimlesh Mann Dr. Kamal Gandhi Dr. P. Narender Raju | Continuing | Rs. 23.72/- |
| 6 | Development of Analytical Tools to Assess Quality of Milk - Protein Based Health Supplements | 2019 - 2022 | IRC (In-house) | Dr. Rajan Sharma (PI) Dr. Kamal Gandhi Dr. Bimlesh Mann Dr. Richa Singh | Continuing | - |
| 7 | Protein profiling of milk from native indigenous breeds (cow and buffalo) in relation to their bioactive potential | 2018 - 2020 | IRC (In-house) | Dr Rajesh Kumar(PI) Dr Bimlesh Mann Dr Rajan Sharma Dr Richa Singh Dr Rajeev Kapila | Continuing | - |
| 8 | Quality evaluation of Butter and Ghee prepared from milk of Indigenous and cross bred cattle. | 2019-2022 | IRC (In-house) | Dr Raman Seth Dr Chand ram Dr Kamal Gandhi | Continuing | - |
- Paper strip based tests for rapid detection of adulterants in milk (sodium chloride, sucrose, neutralizer, urea, glucose, hydrogen peroxide and maltodextrin) were developed. These tests can be done at milk reception as well as at house-hold level.
- Rapid HPLC and TLC based protocols based on tracer component were developed for the detection of vegetable oils/fats adulteration in ghee.
- An analytical protocol was developed for the estimation of vitamin D2 in fortified toned milk.
- A colorimetric test was developed for detection of melamine in milk using Gold nanoparticles.
- Molecular imprinted polymer (MIP) against cephalexin and tetracycline have been developd
- rRapid and semi-quantitative lateral flow based assay methods have been developed for detection of cephalexin and tetracycline in milk.
- A protocol was standardized for the extraction of DNA from ghee as well as from rendered fats (clarified animal body fats).
- A PCR based methodology was developed to detect the pig body fat (lard) in Ghee.
- An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 (AFM1) was designed and developed.
- FTIR based rapid method was developed for assessment of proteolysis in UHT milk during storage.
- A process for preparation of curcumin nanoemulsion optimized using milk proteins and assessed for antioxidant activity in animal model system.
- The stability ofsodium caseinate stabilized curcumin nanoemulsion in Ice-cream was ascertained.
- Nanoemulsions encapsulating clove oil using milk proteins were prepared and assessed for their antimicrobial activity.
- A strategy has been developed for iron fortification of milk and yoghurt using casein hydrolysates-iron complex
- Nano Iron (III) Oxo-Hydroxide Complex fortified biscuits were developed and the organic acid-iron complex was found to be a better iron fortificant with good oxidative stability and bioavailability.
- Methods for preparing milk protein-mineral and lactose-mineral complexes were developed for the fortification purpose.
- Methods were optimized for the preparation of iron-modified milk protein and zinc-modified milk protein complex.
- Whey protein hydrolysates-iron and caseinophorphopeptides-Ca/zn/Fe complexes showed better bioavailability of these elements as compared to their irongemic salt in cell line (Caco-2 cell line) and animal model.
- Antimicrobial peptides were identified from skim milk by fermentation using selected proteolytic lactobacillus strains [αS1-CN (24-34), β-CN (197-209), β-CN (199-209), β-CN (193-207)].
- Casein hydrolysates were evaluated for protection against oxidative stress at different concentrations. It showed no cytotoxic effect and was found to provide cytoprotective effect against H2O2-induced oxidative stress in Caco-2 cells.
- A method for the production of antioxidant peptide rich hydrolysate was standardized by integrating hydrolysis on membrane reactor.
- Buffalo casein hydrolysates enriched with potential antioxidant peptides was d
- Five potential DPPIV inhibitory peptides identified from β-casein (K.YPVEP.F, K.YPVEPF.T, N.LHLPLP.L, N.LHLPLPL.L, L.HLPLPL.L) and one from k-casein (I.NNQFLPYP.Y) following digestion of goat casein under in vitro simulated gastrointestinal conditions.
- A methodology standardized to harvest the fat globules based on their size from cow and buffalo milk. In general, small fat globules fraction contains more than 69% fat globules of size ≤ 2 μM. The medium fat globules fraction contains more than 60% fat globules of size 2 – 4 μM. The large fat globules fraction contains more than 52% fat globules of size > 4 μ.
- A methodology was standardized for characterization of Panchgavya formulation from Indigenous cow "Sahiwal".
- The feasibility of different preservatives like Kathon, Bronopol, Sodium Azide, Sodium Omadine, Dowicil, Triclosan, Hydrogen Peroxide, and Methyl and propyl parabenas as alternative to formalin for preservation of milk stored for analytical purposes was assessed. Bronopol (0.05-0.1%) and kathon (0.20-0.40%) showed better antimicrobial activity.
- A method for preparing whey protein concentrate-iron (WPC-Fe) complex in powder form using spray dryingwas developed. WPC-Fe complex obtained was rich in protein and iron content and could be used as a better and innovative organic iron fortificant with enhanced bioavailability of iron with minimal effect on sensory acceptability, physicochemical characteristics and shelf life, thus alleviating the prevalent iron deficiency.
Head
Dairy Chemistry Division
Email : headdairychemistry[at]gmail[dot]com
Phone : +91-184-2259143
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Page last updated date:21-03-2025 03:35 PM
