Animal Biotechnology

The objectives of the Centre are:

To undertake biotechnology-oriented basic and applied research programmes for improving animal productivity and for developing innovative dairy processes for producing superior quality, safe and wholesome dairy products,
To train manpower for the application of biotechnology for dairy production and dairy processing and
To organize M.Sc./M.V.Sc./M.Tech and Ph.D. programmes in animal biotechnology at NDRI (Deemed University).

Dr. J.K. Kaushik, HOD

Research work in various laboratories at the Animal Biotechnology Centre is focused on three main areas namely embryo biotechnology, animal genomics and proteomics & structural biology. Major areas of interest in embryo biotechnology include in vitro embryo production, animal cloning, production of transgenic animals, embryonic and adult stem cells and cryopreservation of gametes. The Centre holds the distinction of producing world’s first IVF buffalo calf, world’s first buffalo calf produced through Hand-made cloning and India’s first goat kid produced by IVF. The focus of research in animal genomics is on understanding animal reproduction at molecular level, studying the role of miRNAs and remodeling sperm surface for improving reproductive efficiency in buffalo. The Centre has developed PCR-based assays, many of which have been patented. The focus in proteomics is on development of diagnostic kits for early pregnancy detection and identification of subclinical mastitis, and understanding proteomics of milk production and lactation. Another area of interest is to develop novel inexpensive technology for sexing of semen. The structural biology research is aimed at development of bioactive recombinant proteins for human use. In addition to these, the Centre has a laboratory on bioinformatics. Some other areas of research are understanding antibiotic resistance in mastitis-causing bacteria and the use of mesenchymal stem cells for treatment of diseases in dairy animals.

Currently, we have 13 externally-funded research projects funded by DBT, ICAR-NASF, SERB-DST and Bill & Melinda Gates Foundation, USA with the more than 14 crores of funds. Besides these, there are several in-house research projects funded by NDRI. The Centre has the distinction of publishing a large number of research papers every year in scientific journals having high impact factor.

The Centre offers Masters and Doctorate (Animal Biotechnology) degree programs to cater to the need of scientists and researchers in the field of dairy, animal and veterinary sciences in ICAR institutes, agricultural universities and, state and central govt. funded institutions. A summer training program is organized every year to provide on-bench training to post-graduate students of biotechnology from all over India. Currently the Centre has 10 faculty members, 38 M.Sc., M.Tech. and M.V.Sc. students, 75 Ph.D. students, 20 JRFs, SRFs and Research associate, and 4 Post-doctoral researchers and Young Scientists are working at the centre.

I invite you to explore our Institute web site, www.ndri.res.in to learn more about the faculty, research facilities, students, teaching programs and ongoing research projects at the Animal Biotechnology Centre.

For more information, please contact: Dr. S. De
Principal Scientist & Officer In-Charge
Animal Biotechnology Centre
ICAR-NDRI Karnal - 132001
Haryana, India

Sr. No.	Faculty Name	Designation
1	Dr. Jai Kumar Kaushik	Principal Scientist
2	Dr. Dhruba Malakar	Principal Scientist
3	Dr. Manoj Kumar Singh	Senior Scientist
4	Dr. Rakesh Kumar	Principal Scientist
5	Dr. Sachinandan De	Principal Scientist
6	Dr. Satish Kumar	Principal Scientist
7	Dr. Sudarshan Kumar	Senior Scientist

Research activities in animal biotechnology were initiated at NDRI, Karnal during mid-80s with the establishment of a UNDP 'Centre of Excellence on Biotechnology' program in view of the need to utilize biotechnology as a means to transform the agriculture scenario in India. An urgent need for development of biotechnological interventions for improving reproduction and productivity of dairy animals provided the basis for the establishment of Embryo Biotechnology Centre (EBC) at NDRI Karnal by financial support from the Dept. of Biotechnology, Govt. of India. Subsequently, EBC was further strengthened by the establishment of Livestock Genome Laboratory and Molecular Biology Unit under the National Agricultural Research Project-II of ICAR. Animal Biotechnology Centre was reorganized in June 1999 by consolidating all the infrastructure facilities created under various programs on biotechnology at NDRI, Karnal and getting them under one umbrella. A state-of-the-art facility was created in 2007 to consolidate research and teaching activities in biotechnology at the new building of Animal Biotechnology Centre. The new building offers a working space of more than 20,000 sq. ft.

The objectives of the Centre are:

To undertake biotechnology-oriented basic and applied research programmes for improving animal productivity and for developing innovative dairy processes for producing superior quality, safe and wholesome dairy products,
To train manpower for application of biotechnology for dairy production and dairy processing and
To organize M.Sc./M.V.Sc./M.Tech and Ph.D. programmes in animal biotechnology at NDRI (Deemed University).

Master's courses

Sr. No.	Code	Course Title	Aims
1	BT 611	Fundamentals of cell & molecular biology (3+0)	Molecular structure and functions of cells and molecules such as DNA, RNA and proteins.
2	BT 612	Animal cell culture: principles & applications (2+1)	Understanding the principles of animal cell culture and its application
3	BT 613	Immunology applied to biotechnology (2+1)	Understanding the application of immunological techniques in biotechnology.
4	BT 614	Reproductive biotechnology (2+1)	Understanding in-vitro reproductive techniques for ovum and embryo manipulation.
5	BT 621	Applied molecular biology (2+1)	Understanding the principle and application of recombinant DNA in biotechnology.
6	BT 622	Molecular diagnostics (1+2)	Understanding the molecular techniques involved in diagnosis of diseases.
7	BT 623	Animal genomics (2+1)	Understanding structural, functional and comparative genomics of farm animals and its application for livestock improvement
8	BT 624	Techniques in molecular biology & genetic Engineering (0+3)	To provide comprehensive hands-on training on techniques of molecular biology and genetic engineering.
9	BT 629	Master's Seminar (1+0)	-
10	BT 639	Master's Research (20)	-

Ph.D. Courses

Sr. No.	Code	Course Title	Aims
1	BT 711	Gene cloning and expression (3+0)	Understanding the concept of gene cloning and expression.
2	BT 712	Functional genomics & proteomics (3+0)	Understanding gene expression at different conditions/organs
3	BT 713	Advances in reproductive biotechnology (3+0)	Understanding the new developments in reproductive technology.
4	BT 719	Doctoral seminar (1+0)	-
5	BT 721	Trends in Vaccinology (3+0)	Understanding the latest developments in vaccine production technologies.
6	BT 722	Advances in animal cell culture (3+0)	Understanding the latest developments in cell culture techniques.
7	BT 729	Doctoral Seminar II (1+0)	-
8	BT 739	Doctoral Research (20)	-

Our research activities are focused on four major areas namely the embryo biotechnologies, animal genomics, proteomics & system biology and stem cell biology. Faster multiplication of superior germplasms of dairy animals by the application of hand-made cloning technology remains one of the prime areas of our current research. Identification of superior animals utilizing the candidate genes/proteins related to economic and physiological traits of dairy animals ensuring faster genetic gains in animals and development of the selection criteria for dairy animal is the other major area. Fundamental knowledge of one gene synthesizes many proteins is being investigated at the centre to assess the economic traits of dairy animals by utilizing in vitro embryo production system followed by the molecular investigations. Exploring the reproduction traits and disease resistance qualities of Indian dairy breeds are the current interests of the research groups. Proteomics is another branch of the functional genomics, responsible for improvement of dairy production, productivity and processing areas. With the introduction of high-throughput and cutting-edge technology of mass-spectrometry for proteomic studies, the researchers at center are generating the proteomic profile of sub-clinical mastitis and matritis diseases. Early detection of pregnancy utilizing PAGs and miRNA are also being investigated at the center. Development of recombinant proteins as the intermediate technologies for fulfilling the needs of the animal and dairy industry forms another major area of research at ABTC. Many such useful recombinant proteins such as LIF, GDF-9, Lectoferrin, SSLP and amino-peptidases have been developed in the recent past. Researchers at the centre are also engaged in promoting development of designer animals and humanized dairy milk from animals by utilizing CRISPER and TALEN based genome editing tools. In the era of high usage of antibiotics in dairy industry, stem cell technology has emerged as an alternative to treatment with antibiotics for diseased udder, skin and hoof wounds. Mesenchymal stem cell technology has been developed at the centre and routinely utilized for treatment of animal diseases.

The state of art laboratories equipped with modern and high end equipments and tools are available at ABTC to carry out all research activities. The laboratories are equipped with equipments like LC-MS/MS, Flow cytometer with sorter, Bioreactors, DNA sequencer, Real-time PCR, Micromanipulator and range of incubators and freezers. A specialized bioinformatic facility is available to cater the need of researchers for studying the niche areas of bioinformatics related to animal biotechnology.

Patents

A non-invasive DNA isolation methodology in Dairy Animals. (Application No-1366/Del/2007). Patent Grant Date: 31-01-2014.
A PCR based method for differentiating Cow and Buffalo milk (No-011-020181 dated 22/11/2013).

Technologies

DNA based method for differentiation of cow & buffalo meat, ghee, butter, milk and milk products.
Universal cell lysis solution for direct PCR from animal, plant tissues and bacterial cells.
A PCR (polymerase Chain Reaction) based A1/ A2 beta casein genotyping method for milk and milk product.
Development of mesenchymal stem cells from adipose issues for treatment of wound, paralysis, fracture, mastitis, endometritis and cancer in animals.
Generation of oocytes from embryonic and induced pluripotent stem cells.

List of externally funded projects (as on March 6, 2019)

Sr. No.	Project Name	Funded By	Duration	Project Leader
1	Deciphering the circulating miRNAs from terminal stage pre-implantation embryos and placentomes for early detection of pregnancy in buffalo	DBT, Govt. of India: Rs. 52.2715 lakhs	4.8.2016 to 3.7.2019	Dr. T. K. Datta, Co-Leaders : Dr. Rakesh Kumar and Dr. A. Kumaresan
2	Synthesis, characterization and effect of graded levels of nano-selenium supplementation on the performance of broiler chicken	ICAR-NASF: Rs. 42.727 lakhs	1.6.2017 to 31.5.2020	Dr. A.K. Mohanty, Co-Leaders : Dr. Sudarshan Kumar
3	Genetic variability of milk protein and its characterization by proteomic approach in Indian goats	ICAR-NASF: Rs. 34.45080 lakhs	1.6.2017 to 31.5.2020	Dr. S. De
4	Conservation of Indigenous Pig of Assam through Handmade Cloning Technique	DBT, Govt. of India: Rs. 40.59 lakhs	2.1.2017 to 1.1.2020	Dr. M. K. Singh
5	Global transcriptome and miRNA analysis for deciphering reasons for low cloning efficiency in buffalo	SERB, DST, Govt. of India: Rs. 46.78 lakhs	22.3.18 to 21.3.21	Dr. P. Palta, Co-Leaders : Dr. M. K. Singh
6	Production of multiple copies of elite buffalo bulls using animal cloning technology	ICAR-NASF: Rs. 264.2964 lakhs	31.3.2018 to 30.3.2022	Dr. P. Palta, Co-Leaders : Dr. M. K. Singh, Dr. S. S. Lathwal, Dr. Subhash Chand
7	Genome editing of MFGE8 and S100 genes in bovine mammary epithelial cells to understand their role in milk production	SERB, DST, Govt. of India; Rs. 62.538 lakhs	21.3.2018 to 20.3.2021	A. K. Mohanty, Co-Leaders : Dr. Sudarshan Kumar, Dr. J. K. Kaushik, Dr. Malakar, Dr. Satish Kumar
8	Improving the usability of buffalo spermatozoa by sperm surface remodelling and immune acceptance in female reproductive tract	ICAR-NASF: Rs. 174.60 lakhs	1.7.2018 to 30.7.2021	Dr. T. K. Datta, Co-Leaders : Dr. Rakesh Kumar, Dr. J. K. Kaushik
9	Molecular Markers for Improving Reproduction of Cattle and Buffaloes	Bill & Melinda Gates Foundation, USA: Rs. 648.38 lakhs	31.07.2018 to 31.07.2023	Dr. T. K. Datta, Co-Leaders : Dr. Rakesh Kumar, Dr. Dheer Singh, Dr. Suneel Onteru, Dr. R. K. Baithalu, Dr. A. K. Mohanty, Dr. Sudarshan Kumar, Dr. T. K. Mohanty
10	Targeted immobilization of Y-bearing spermatozoa and modulation of oviduct milieu for skewing sex ratio towards female offspring in dairy cattle	ICAR-NASF: Rs. 11.34 lakhs	01.08.2018 to 31.07.2021	Dr. Rakesh Kumar, Co-Leaders : Dr. T. K. Datta
11	CRISPR/CAS9 guided functional analysis of genes regulating early embryonic survival in buffalo	ICAR-NASF: Rs. 62.4431 lakhs	01.08.2018 to 31.07.2021	Dr. Dhruba Malakar, Co-Leaders : Dr. Satish Kumar
12	Development of early pregnancy diagnostic assay through discovery of biomarkers in cattle and buffalo	DBT, Govt. of India: Rs. 114.70725 lakhs	10.5.2018 to 9.5.2021	Dr. A. K. Mohanty, Co-Leaders : Dr. R. K. Baithalu, Dr. T. K. Mahanty, Dr. Sudarshan Kumar
13	Semen sexing in cattle	ICAR: Rs. 239.5 lakhs	1.4.2017 to 31.3.2021	Dr. A. K. Mohanty, Co-Leaders : Dr. Sudarshan Kumar

PDF Projects

Sr. No.	Project Name	Project By	Duration Year	Project Workers
1	Quantitative profiling of buffalo milk fat globule membrane, a promising nutraceutical for infant formula milk	Woman Scientist Scheme of DST)	2017-20	A. Turan, J. K. Kaushik
2	Profiling of milk proteome in different species of farm animals and comparative evaluation of host defense proteins	NPDF project	2016-18	A. Chopra, A. K. Mohanty
3	Protein profiling of semen of cross-bred cattle bulls for identification of biomarkers for high semen freezability	SERB-NPDF	2017-2019	S. Kalra, A. K. Mohanty
4	Pesticide exposure and sperm fertilizing ability: A study on Bovine Model	SERB-PDF	2018-20	Shivani Chhillar and T. K. Datta
5	Delineating genome wide methylation dynamics in cows of native and exotic origin: An approach to understand heat stress adaptation	SERB-PDF	2018-20	Preeti Verma and A. K. Mohanty
6	Development of methods for detection of betacasomorphin-7 in biological samples, (urine or blood) by aptamers	ICMR-RA	2018-21	Abhishek and A. K. Mohanty

A male cloned calf named 'Rajat', was born on July 23, 2014 by normal parturition. Rajat was produced through Hand-guided cloning using somatic cells isolated from the frozen-thawed semen of a highly ranked progeny-tested Murrah buffalo bull (MU-4393) which had died many years back.
A female calf named 'Deepasha' was born on December 12, 2014 by normal parturition. Deepasha is a clone of a wild buffalo named 'Asha', that has been kept in semi-captivity at Chhattisgarh. The wild buffalo is an endangered species.
A female cloned calf named 'Apurva' was born on February 5, 2015 through normal parturition. Apurva was produced using somatic cells isolated from urine.
A female cloned calf named 'Lalima' was born on May 2, 2014 by normal parturition. Lalima is a clone of an elite Murrah buffalo (MU-5345) of NDRI Livestock Farm.
Garima II, the cloned buffalo, delivered second female calf named 'Karishma' on December 27, 2014 through normal parturition.
A female cloned calf named 'Swarupa' produced through 'Hand-guided Cloning' was born on August 1, 2015. Swarupa is a clone of 'Karan-Kirti', the highest milk producing Murrah buffalo at NDRI Livestock Research Centre.
Transgenic buffalo and goat embryos containing human insulin gene were produced from transfected buffalo fetal fibroblasts.
Germ line cell-specific genes were identified in buffalo and buffalo embryonic stem cell lines were differentiated towards germ lineage.
VASA-transgenic buffalo embryonic stem cell lines were established and differentiated to germ lineage.
Global transcriptome profiling was carried out in goat embryos at different stages of development, produced by in vitro fertilization, somatic cell nuclear transfer and parthenogenesis.
Buffalo recombinant interferon-tau was produced.
Cloning work at NDRI was highlighted as 9^th among top 20 science stories of 2014 by 'Scientific India', a science magazine.
Cloned buffalo embryos were successfully produced using trophoblast cells, urine and milk-derived somatic cells and lymphocytes as donor cells.
Cloned blastocysts derived from fibroblasts, milk-derived cells, lymphocytes, trophoblast cells and those produced by in vitro fertilization were found to differ in their developmental competence, level of apoptosis, epigenetic status and expression level of many important genes.
Buffalo trophoblast cell lines were developed from blastocysts produced by in vitro fertilization, Hand-guided cloning and parthenogenesis. A feeder-free in vitro culture system was developed which enabled their long-term culture.
Treatment of buffalo donor cells with epigenetic modifiers was shown to improve the blastocyst rate and quality of cloned embryos
Cloned female and male buffalo embryos were found to differ in their developmental competence, epigenetic status, sex-biased transcription patterns in X-linked genes and response to epigenetic modifiers. Aberrant X-linked gene expression occurred frequently in female embryos.
Transgenic cloned buffalo embryos were produced containing pAcGFP-buSCD, a mammary gland specific expression vector, which is able to convert high level of conjugated linoleic acid in mammary gland epithelial cells.
Global transcriptome and miRNA profiling was carried out in buffalo blastocyst-stage embryos produced by cloning and in vitro fertilization techniques to identify the shortcomings with cloned embryos. This will help in making strategies for improving the success rate of producing cloned calves in cattle and buffaloes.
Transgenic buffalo spermatogonial stem cells were produced and used for homologous transplantation.
Mammary lineage cells were produced by directed differentiation of buffalo embryonic stem cells.
Novel non-coding RNAs were discovered in buffalo oocytes which may influence their development competence.
Recombinant bovine lukemia inhibitory factor was produced successfully. Its use as a supplement for culture of bovine stem cells may help improve efficiency and reduce the cost.
Direct application of mesenchymal stem cells isolated from adipocyte tissue was demonstrated to be effective in management of chronic hoof wounds in cattle and buffaloes.
Treatment of human intestinal cells with recombinantly produced lactobacilli surface layer proteins could prevent pathogen binding to the extent of as high as 76%.
Application of pulsed electromagnetic field to reconstructed embryos during Hand-guided cloning was shown to increase the blastocyst production rate and improve the quality of cloned embryos produced.
Transgenic goat embryos, having GFP integration at Rosa 26 locus, were produced using one of the latest genome editing tools Transcription Activator-like Effector Nucleases (TALEN).
Tetraploid complementation with putative parthenogenetic embryonic stem cells was successfully used for production of chimeric blastocyst-stage goat embryos.
Treatment of cloned embryos with miR-145 inhibitor was shown to improve their developmental competence and quality, and increase histone acetylation and expression level of several pluripotency-related genes.
A novel non-coding RNA (BOA 290U) was identified in buffalo oocyte and its association was established with expressed proteins. It is potentially important for imparting developmental competence to oocytes.
The chronology of GVBD event was found to be associated with competence of buffalo oocytes. Holding oocytes temporarily at GV stage was found to be useful in enhancing their developmental ability.
High throughput RNA sequencing of buffalo oocytes revealed genes which are significantly affected in 'Good' vs. 'Bad' oocytes. Affected pathways in inferior quality oocytes were identified.
A battery of unique non-coding mi & pi RNAs was discovered in buffalo oocytes, which could be linked to their competence. Major genes under CLR and NLR category were amplified and sequenced completely.
Basal expression pattern of important RLR, NLR and CLR genes was compared in immune and non-immune buffalo tissues. A large number of splice and transcript variants were observed to be present under RLR, NLR and CLR category in different types of buffalo tissues.
Potential protein biomarkers have been identified in urine and serum of cows for early detection of pregnancy 16 days post artificial insemination.
A proteomics-based method was developed for detection of A1/A2 milk of cattle and buffaloes
Candidate microRNAs, which were uniquely expressed during early pregnancy (30 days) in buffalo, were identified.
Specific miRNAs associated with maternal and fetal placentomes were identified in early pregnant buffaloes. A step towards detection of pregnancy by miRNAs.
Comparative sequence analysis of β-defensins from different species by bioinformatic analysis revealed that buffalo β-defensins have unique conserved functional motifs although their sequences are quite different from those of other closely related mammals.
Increased expression of sperm coat protein, beta-defensins 126 and 129 was detected in epididymis of buffalo testes.
Eight novel variants of goat kappa casein variants were revealed from seven different Indian goat breeds.
Functional characterization of Milk fat globule membrane epidermal growth factor 8 was performed by gene silencing using shRNA. It was observed that MFGE8 is essential for maintenance of cell shape and morphology during lactation in farm animals.
A total of 1600 proteins were identified in bovine urine by using LC-MS/MS for the first time. Various methods were optimized for extraction of proteins from bovine urine.
Three potential urine protein biomarkers (bovine allergen, Mannan-binding lectin serine peptidase 2, Alpha-1-microglobulin, Glutaminyl-peptide cyclo-transferase) were identified for detection of early pregnancy (~ 20 days) in cows. Three proteins namely vitamin-binding protein, apolipoprptein A and complement 3 were up-regulated in bovine serum during pregnancy (16^th to 45^th day).
Differential transcript analysis in fetal cotyledon of early pregnant buffalo (~2 months) revealed that prostate-specific antigen is over-expressed during early pregnancy.
Recombinant leucyl aminopeptidase of Lactobacillus species was expressed in biologically active form.
Buffalo hepatocytes were successfully cultured for over a week on several extracellular matrices and feeder fibroblast cells.
Methods were standardized for in vitro differentiation of buffalo mammary epithelial cells for expression of milk proteins (casein).
The function of MGP-40, a mammary-specific protein which protects the loss of mammary epithelial cells during mammary gland involution via activation of STAT-3 phosphorylation, was deciphered.
Buffalo oviductin was observed to have a positive effect on several sperm quality-related parameters such as motility, viability, acrosomal integrity and capacitation. Oviductin was found to increase cleavage rate and blastocyst formation during IVF.
Salivary proteome of Sahiwal cows revealed 3000 proteins, which is the highest number reported hitherto.
A stably transfected COS-1 cell line was established which expressed recombinant buffalo leukemia inhibitory factor.
Recombinant buffalo GDF9 (57 kDa) was shown to enhance cleavage and blastocyst rate during production of IVF buffalo embryos.
Recombinant mucus binding protein (rMub), S-layer protein (rSlp) of lactic acid bacteria were shown to preclude the binding of enterotoxigenic coli (ETEC) with human enteric cell lines Caco-2 and HT-29.
Recombinant leucyl aminopeptidase and prolyl aminopeptidase of lactobacilli origin were expressed, their physicochemical properties studied and their specific activity was shown to be better or comparable to that of commercially available Corolase enzyme.
Proteins differentially expressed in milk whey of A1 and A2 milk were identified for analyzing their health implications
New definition of precious pashmina fibers was established with its unique proteomic compositions. Key proteins involved in Pashmina fibre growth and development were identified by analysis of Pashmina fibre and skin proteome.Around 7000 proteins were identified in the skin proteome of Pashmina goats
A proof-of-concept was developed for the detection of pregnancy in cattle, as early as 35 days, using an antibody against Pregnancy Associated Glycoprotein-7 (PAG7).
A LC-MS method was developed for identification of A1 and A2 beta caseins in milk of Karan Fries and Sahiwal cattle. The method has been validated in more than 200 cows.
More than 6800 proteins were identified for the first time in milk of Sahiwal cattle.
1371 differentially expressed genes and 757 differentially expressed proteins were identifies during lactogenic differentiation in buffalo mammary epithelial cells and their possible role in lactation was studied.
A stably transfected COS-1 cell line was developed which expresses recombinant bovine Leukemia Inhibitory Factor .
Mesenchymal stem cells used successfully to permanently cure hoof wounds in cattle and buffaloes.
Mouse allogenic and cattle xenogeneic mesenchymal stem cells were used to successfully heal fractured tibial bones of mice within 5 weeks.
Recombinant buffalo sperm lysozyme like protein 5 (SPACA5) was produced using coli expression system.
More than 12000 proteins have been profiled in Buffalo mammary epithelial cells, and 6 novel proteins were found to be involved in lactogenesis.
Three different isoforms of buffalo pregnancy associated glycoproteins (PAG-1, PAG-2 & PAG-18) were produced in coli expression system.
An innovative microfluidic device was developed for partial enrichment of live and motile sperm cells.
Mesenchymal stem cells were successfully used to cure mastitis and metritis in cattle.

Sr. No.	Name of the Student Passed Out	Guide	MSc/MVSc/Mtech	Year Passed Out	Currently at	Email
1	Mr. S. K. Das	S L Goswami	MSc	1991	-	subratakdas1[at]gmail[dot]com
2	Ms. H. S. Lalitha	S L Goswami	MSc	1996	-	-
3	Ms.Parul Mann	S L Goswami	MSc	1996	-	-
4	Mr. Imtiaz Zafar	S L Goswami	MSc	2000	-	imtiaz[dot]ncbs[at]gmail[dot]com
5	D. Vasanth	D. Malakar	M. Sc.	2004	Guest Faculty at the Department of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu	vasanthguy[at]gmail[dot]com
6	Aravindhan G. N.	T K Datta	M.V.Sc.	2004	-	arvindhan[dot]nagarajan[at]yale[dot]edu
7	Ms. Kamna Singh	S L Goswami	MSc	2004	-	-
8	Ajay Pal Singh Aswal	T K Datta	M.V.Sc.	2005	-	aswal_aps[at]rediffmail[dot]com
9	Dr. Sudarshan Kumar	J. K. Kaushik	MVSc	2005	ARS Scientist, Animal Biotechnology Centre, National Dairy Research Institue, Karnal	kumarsudershan[at]gmail[dot]com
10	Dr. Sachin S. Pawar	D. Malakar	M.V. Sc.	2006	Scientist (Animal Biotechnology) National Institute of Abiotic Stress Management Malegaon Baramati 413 115, District Pune	sachinndri[at]gmail[dot]com
11	Dr. Arun Kumar De	D. Malakar	M. V. Sc.	2007	Scientist (Animal Biotechnology) ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobor	biotech[dot]cari[at]gmail[dot]com
12	Aditya Prasad Sahoo	T K Datta	M.V.Sc.	2007	Scientist, National FMDCP Seromonitoring Laboratory, ICAR-DFMD, Bengaluru	rush2aditya[at]gmail[dot]com
13	Ms. Moloya Gohain	S L Goswami	MSc	2008	-	moloyandri[at]gmail[dot]com
14	Dr. Anoop J.	J. K. Kaushik	MVSc	2008	Manager, KLD Board, Mattupatty, Munnar, Idukki, Kerala	janoop73[at]gmail[dot]com
15	Dr. Manoj Kumar Jena	D. Malakar	M. V. Sc.	2009	Assistant Professor, Department of Biotechnology School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar, Punjab	drmanoj[dot]jena[at]gmail[dot]com
16	Kumari Arpana Verma	T K Datta	M.V.Sc.	2009	ICMR Postdoctoral fellow NIRRH,Mumbai,India	arpanandri[at]gmail[dot]com
17	Mr. Guru Prasad	S L Goswami	MSc	2009	-	-
18	Dr. Rahul Dutta	D. Malakar	M. V. Sc.	2010	Grants Adviser, Well come/DBT India Alliance, Hyderabad	doctordut[at]gmail[dot]com
19	Sandeep Kumar	T K Datta	M.V.Sc.	2010	Senior Scientist, Xcelris Labs Ltd., Ahmedabad	sandeepndribiotech[at]gmail[dot]com
20	Gattu Rudrappa	J. K. Kaushik	MSc	2010	PhD Scholar, IOB, Bangalore	bio[dot]rudra[at]gmail[dot]com
21	Gadave Kaustubh Subhash	J. K. Kaushik	MVSc	2010	Serum Institute of India Pvt Ltd, Pune, Maharashtra	kaustubhvet[at]gmail[dot]com
22	Mata Naga Raja	D. Malakar	M. Sc.	2011	-	-
23	Shashi Anand	T K Datta	M.Sc.	2011	Postdoctoral Fellow, Mitchell Cancer Institute, University of South Alalbama, USA	shashianand15[at]gmail[dot]com
24	Sohita Ojha	S L Goswami	MSc	2011	-	samaira[dot]sohita[at]gmail[dot]com
25	Manoj Kumar	J. K. Kaushik	MVSc	2011	Veterinary Officer, Deptt of Animal Husbandry, Govt of Bihar, Kundra, Kaimur, Bihar	manoj2003vet[at]gmail[dot]com
26	Nisha Bara	D. Malakar	M.Sc.	2012	-	nishabara5432[at]gmail[dot]com
27	Sirlapu Madhusudan Rao	T K Datta	M.Sc.	2012	Senior consultant Embryologist at Pearl Singapore Fertility centre and Research Institute, chennai., and Guest teaching faculty at Asia Pacific Institute of Embryology, Mysore.	madhuvennela7[at]gmail[dot]com
28	Subas Chandra Jena	T K Datta	M.V.Sc.	2012	Additional Veterinary Assistant Surgeon (AVAS), Malkangiri,Dept. of Fisheries and Animal Resources Development Dept(F&ARD), Govt Of Odisha	drsubasvet[at]gmail[dot]com
29	Chanukuppa Venkatesh	J. K. Kaushik	MSc	2012	PhD Scholar, Mass spectrometry and proteomics lab, National Centre for Cell Science, NCCS complex, SPPU Campus, PO-411007	venkatesh[dot]ch53[at]gmail[dot]com
30	Mona Faraji Heriss	T K Datta	M Sc.	2013	-	monamonafar[at]gmail[dot]com
31	Amit Dubey	D. Malakar	M.Sc.	2013	Doing Ph. D. at Canada	dubeyamit786[at]gmail[dot]com
32	Kaleem Ahmed	J. K. Kaushik	MSc	2013	-	kaleem0598[at]gmail[dot]com
33	Samreen Fatima	D. Malakar	M.Sc.	2014	Completed her Ph. D. at JNU, India	asad[dot]samreen[at]gmail[dot]com
34	Mr. Banshi Nath	M.K. Singh	M.Sc.	2014	Ph.D. (Pursuing at University Laval, Quebec Canada)	banshi[dot]nath[dot]1[at]gmail[dot]com
35	Syed Mohd. Aamir Suhail	J. K. Kaushik	MSc	2014	PhD Scholar, Regional Centre for Biotechnology, Faridabad, Haryana	aamirbkbz[at]yahoo[dot]com
36	Miss. Vanya Bhushan	D. Malakar	M. Sc.	2015	Doing Ph.D. at NDRI, Karnal	bhushan[dot]vanya22[at]gmail[dot]com
37	Mr. Anurag Kumar	M.K. Singh	M. Tech.	2015	Ph.D. (Pursuing at NDRI, Karnal)	anuragkumarbt[at]gmail[dot]com
38	Gaurav Kumar Chaubey	T K Datta	M Sc.	2016	Ph.D. Student, CSIR-IMTECH, Chandigarh	gchaubey93[at]gmail[dot]com
39	Ms. Kamlesh Kumari Bajua	D. Malakar	M. Sc.	2016	Doing Ph. D. at NDRI, Karnal	kamleshafs123[at]gmail[dot]com
40	Mr. Manish Tiwari	M.K. Singh	M. Tech.	2016	Ph.D. (Pursuing at NDRI, Karnal)	bt[dot]manish1[at]gmail[dot]com
41	Madhisamita Panda	Rakesh Kumar	M.V.Sc.	2016	Veterinary Officer, State of Orissa	madhusmita[dot]biotech24[at]gmail[dot]com
42	Vibhanshu Kumar	J. K. Kaushik	MSc	2016	PhD Scholar, IISER, Bhopal	vibhu[dot]doctor[at]gmail[dot]com
43	Mr. Avinash Dwivedi	M.K. Singh	M. Tech.	2017	-	newavi[dot]gkp[at]gmail[dot]com
44	Vinay Malik	Rakesh Kumar	M.Tech	2017	Residing in Shamli, UP.	lifescience39[at]gmail[dot]com
45	Ms. Haphidasara Pyngrope	J. K. Kaushik	MSc	2017	Junior Research Fellow, Dept. of ENT, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, Meghalaya, 793018.	haphidasara58[at]gmail[dot]com
46	Diptesh Das	Rakesh Kumar	MSc	2017	Pursuing Ph.D. at NDRI, Karnal	dipteshdas[at]outlook[dot]com
47	Ms. Amina Ambareen	J. K. Kaushik	MTech	2017	-	ameena_1633[at]outlook[dot]com
48	Sakshi Khurana	T K Datta	M Sc.	2018	Ph.D. Student, HAIC Integrated Mushroom Research & Development Center (lab), DCRUST,Murthal	sakshi[dot]khurana92[at]gmail[dot]com
49	Pratiksha Dubey	T K Datta	M Sc.	2018	Ph.D. Student, IISER, Mohali, Punjab	pratiksha[dot]du[at]gmail[dot]com
50	Suman Tamang	T K Datta	M Sc.	2018	-	sumsthinker[at]gmail[dot]com
51	Apoorva Soni	T K Datta	M Sc.	2019	Junior Research Fellow, TMC-ACTREC, Navi Mumbai, India	soniapoorva7[at]gmail[dot]com
52	Ramandeep Singh	D. Malakar	M. Sc.	2019	Completed M. Sc. at NDRI, Karnal	docrd95[at]gmail[dot]com
53	Komal Dagar	Rakesh Kumar	MSc	2019	Residing in Delhi	komaldagar1605[at]gmail[dot]com
54	Ms. Akansha Bhatt	J. K. Kaushik	MTech	2019	PhD Scholar, AIIMS, Delhi	akanshajmd1[at]gmail[dot]com
55	Vinay Bhaskar	D. Malakar	M. Sc.	2020	Doing M. Sc. at NDRI, Karnal	vinaybhaskar99[at]gmail[dot]com
56	Ms. Navkiran Kaur	J. K. Kaushik	MSc	2020	-	navkirannavy[at]gmail[dot]com
57	Ms. Jyoti Yadav	J. K. Kaushik	MSc	2020	-	yjyoti039[at]gmail[dot]com